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目的:探讨急性ST段抬高性心肌梗死(STEMI)患者与正常人血浆纤维蛋白原(Fg)含量的表达差异及Fg与急性STE-MI发生、发展的关系。方法:选取我院确诊为急性STEMI的患者血浆32例为急性STEMI组,同期行急性心肌梗死(AMI)筛查无异常的正常人血浆32例为对照组。应用蛋白质免疫印迹技术(Western Blot)及酶联免疫吸附法(ELISA)验证Fg表达差异。结果:Western Blot法测定急性STEMI组血浆Fg灰度值为(0.933 3±0.256 8),对照组为(0.786 0±0.203 6),两组比较差异有统计学意义(t=3.354,P<0.01)。ELISA法测定急性STEMI组Fg含量为[(14.202 1±3.913 0)g/L],对照组为[(3.315 0±0.891 5)g/L],两组比较差异有统计学意义(t=4.525,P<0.01)。结论:急性STEMI组患者的Fg水平明显高于对照组,提示Fg可能对急性STEMI的发生、发展起到促进作用。
Objective: To investigate the difference of plasma fibrinogen (Fg) expression in patients with acute ST-segment elevation myocardial infarction (STEMI) and normal persons and the relationship between Fg and the occurrence and development of acute STE-MI. Methods: Thirty-two patients with acute STEMI were selected as the acute STEMI group. Thirty-two normal subjects without acute myocardial infarction (AMI) were selected as the control group. Fg expression was verified by Western Blot and enzyme-linked immunosorbent assay (ELISA). Results: The plasma Fg value of the acute STEMI group was (0.933 3 ± 0.256 8) and that of the control group (0.786 ± 0.203 6) by Western Blot. There was significant difference between the two groups (t = 3.354, P <0.01) ). The Fg content in acute STEMI group was [(14.202 1 ± 3.913 0) g / L] and [(3.315 0 ± 0.891 5) g / L] in the control group by ELISA. There was significant difference between the two groups , P <0.01). Conclusion: Fg levels in patients with acute STEMI were significantly higher than those in control group, suggesting that Fg might promote the occurrence and development of acute STEMI.