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目的 探讨胃癌及其癌前病变线粒体DNA微卫星不稳定 (mtMSI)与Bcl 2、BaxmRNA表达的关系。方法 采用PCR SS CP方法检测mtMSI,并采用RT PCR方法检测了Bcl 2和BaxmRNA的表达。结果 胃黏膜肠化 (5 3 3% )、异型增生 (70 % )组织Bcl 2mRNA表达率显著高于浅表性胃炎组(10 % ) (P <0 0 5 ) ,而慢性萎缩性胃炎 (5 0 % )和胃癌 (30 % )Bcl 2mRNA表达率与浅表胃炎组相比差异无统计学意义 (P >0 0 5 ) ;胃黏膜异型增生组织Bcl 2mRNA表达率显著高于胃癌组织 (P <0 0 5 )。异型增生 (6 0 % )组BaxmRNA表达率显著高于浅表胃炎组 (P <0 0 5 ) ,而慢性萎缩性胃炎(5 0 % )、肠化 (4 6 7% )和胃癌 (33 3% )的BaxmRNA表达率与浅表性胃炎组 (10 % )比较差异无统计学意义 (P >0 0 5 )。mtMSI(+)组Bcl 2mRNA表达率为 5 0 % ,mtMSI(- )组为 4 0 5 % ;mtMSI(+)组BaxmRNA表达率为 4 4 4 % ,mtMSI(- )组为 4 0 5 % ,mtMSI(+)组Bcl 2和BaxmRNA表达率与mtMSI(- )组相比差异无统计学意义 (P >0 0 5 )。结论 mtMSI可能在部分胃癌的发生中起重要作用 ;胃癌发生过程中出现的mtMSI与Bcl 2、BaxmRNA的异常表达可能无关
Objective To investigate the relationship between mitochondrial DNA (mtMSI) and the expression of Bcl-2 and Bax mRNA in gastric cancer and precancerous lesions. Methods mtMSI was detected by PCR SSCP method and the expression of Bcl-2 and Bax mRNA was detected by RT-PCR. Results The rate of Bcl-2 mRNA expression in gastric mucosal intestinal metaplasia (53.3%) and dysplasia (70%) was significantly higher than that in superficial gastritis (10%) (P <0.05), while chronic atrophic gastritis There was no significant difference in the expression of Bcl-2 mRNA in gastric cancer (0%) and gastric cancer (30%) compared with superficial gastritis group (P> 0.05). The expression of Bcl-2 mRNA in gastric dysplasia was significantly higher than that in gastric cancer (P < 0 0 5). The rate of Bax mRNA expression in dysplasia (60%) group was significantly higher than that in superficial gastritis group (P <0 05), while chronic atrophic gastritis (50%), intestinal metaplasia (46.7% %) Had no significant difference in the expression of Bax mRNA between the superficial gastritis group and the superficial gastritis group (10%) (P> 0.05). The expression rate of Bcl-2 mRNA in mtMSI (+) group was 50%, that in mtMSI (-) group was 45.5%, that in mtMSI group was 44.4%, that in mtMSI group was 40.5% The expression of Bcl-2 and Bax mRNA in mtMSI (+) group was not significantly different from that in mtMSI (-) group (P> 0.05). Conclusion mtMSI may play an important role in the development of gastric cancer. The abnormal expression of mtMSI, Bcl-2 and Bax mRNA in gastric carcinogenesis may not be related