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野生大豆Glvcine soja是栽培大豆G.max的近祖。它具有高蛋白、抗病性强、抗旱、抗涝和抗盐碱等许多优良性状。研究G.soja的愈伤组织的再生,对于研究Glycine属的遗传工程,改良现有栽培大豆都有重要意义。本文介绍采用MS基本培养基附加2mg/l IAA、5mg/l BA和2mg/l KT培养基,培养未成熟的G.soja幼嫩种子的下胚轴和子叶获得愈伤组织并分化出幼苗。 IAA——Indole—Acetic Acid. BA——6—Benzylamiaopurine. KT——Kinetin.
Wild soybean Glvcine soja is the ancestor of cultivated soybean G. max. It has high protein, disease resistance, drought, waterlogging and salt and many other good traits. Research on regeneration of G. soja callus is of great significance for studying the genetic engineering of Glycine genus and improving existing cultivated soybean. This article describes the addition of 2mg / l IAA, 5mg / l BA and 2mg / l KT medium with MS basal medium, hypocotyls and cotyledons of young immature G.soja seed, callus induction and differentiation of seedlings. IAA - Indole-Acetic Acid. BA - 6-Benzylamiaopurine. KT - Kinetin.