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目的:观察水蛭对肺纤维化大鼠PAI-1的作用,并探讨其机制及意义。方法:采用气管内注入博来霉素制作大鼠肺纤维化模型,成膜后随机分为两组,模型组(BM组)和水蛭组(HD组),另设鼠龄、体重相匹配的正常对照组(NC组)。28天后ELISA法测定血浆PAI-1含量,并测定其活性,同时检测肺组织羟脯氨酸(HYP)含量。结果:NC组PAI-1含量及活性明显低于其它各组,BM组PAI-1含量及活性高于HD。NC组、HD组HYP低于BM组。结论:水蛭可能通过减少凝血酶在肺内的表达,抑制PAI-1生成及活性,使u PA活性升高,减少纤维蛋白沉积,对肺纤维化大鼠肺组织具有保护作用。
Objective: To observe the effect of leeches on PAI-1 in rats with pulmonary fibrosis and to explore its mechanism and significance. Methods: Pulmonary fibrosis model was established by intratracheal injection of bleomycin. The rats were randomly divided into two groups: model group (BM group) and leech group (HD group) Normal control group (NC group). After 28 days, the content of PAI-1 in plasma was measured by ELISA, and the activity of hydroxyproline (HYP) in lung tissue was measured. Results: The content and activity of PAI-1 in NC group were significantly lower than those in other groups. The content and activity of PAI-1 in BM group were higher than those in HD group. The HYP in NC group and HD group was lower than that in BM group. Conclusion: Leech may protect the lung tissue of rats with pulmonary fibrosis by reducing the expression of thrombin in the lung, inhibiting the formation and activity of PAI-1, increasing the activity of u PA, reducing fibrin deposition.