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目的：了解Ｂ７１ 分子在体外抗肝癌免疫反应中对自然杀伤细胞（ＮＫ 细胞） 杀伤活性的影响。方法：经脂质体ＤＯＳＰＥＲ 介导将ｈＢ７１ 基因直接导入ＨｅｐＧ２ 细胞，建立ＨｅｐＧ２／ｈＢ７１ 细胞克隆。分离健康人和肝癌患者外周血淋巴细胞，以ＭＴＴ 法测定ＮＫ 细胞对ＨｅｐＧ２／ｈＢ７１ 细胞的杀伤活性，以杀伤ＨｅｐＧ２／ｎｅｏ 和ＨｅｐＧ２ 细胞为对照。结果： ＨｅｐＧ２ 细胞转染ｈＢ７１ 基因后表达Ｂ７１ 分子。无论在健康人组还是肝癌患者组， 健康人外周血ＮＫ 细胞杀伤ＨｅｐＧ２／ ｎｅｏ ， ＨｅｐＧ２ 细胞的活性约为肝癌患者ＮＫ 活性水平的２ 倍。健康人和肝癌患者外周血ＮＫ 细胞对ＨｅｐＧ２／ｈＢ７１ 细胞的杀伤活性均较对照组明显增高（ 增强１－４９～３－４８ 倍） ， 对ＨｅｐＧ２／ ｈＢ７１ 细胞的杀伤活性在Ｅ／ Ｔ＝ ５ ∶１时分别为５７－２ 及４３－８ ，有显著性差异（ Ｐ＜ ０－０５） ，在Ｅ／Ｔ＝ １０ ∶１ 及２０ ∶１ 时分别可达８２－８ ～８４－４ 和７８－６ ～８６－４ ，差异不显著（ Ｐ ＞ ０－０５） 。结论：Ｂ７１ 分子显著增强ＮＫ 细胞对肝癌细胞的体外杀伤活性，可能通过激活非特异性细胞免疫在抗肝癌复发中发挥作用。
Objective: To investigate the effect of B71 molecule on killing activity of natural killer (NK) cells in vitro against hepatocellular carcinoma immune response. METHODS: The hB71 gene was directly transfected into HepG2 cells via liposome dosperm and a HepG2/hB71 cell clone was established. The peripheral blood lymphocytes of healthy and liver cancer patients were isolated. The killing activity of NK cells against HepG2/hB7 1 cells was measured by MTT assay, and the HepG2/neo and HepG2 cells were killed as controls. Results: HepG2 cells transfected with hB71 gene expressed B71 molecule. In healthy individuals and patients with hepatocellular carcinoma, peripheral blood NK cells kill HepG2/neo, and the activity of HepG2 cells is about 2 times the NK activity level of liver cancer patients. The killing activity of peripheral blood NK cells against HepG2/hB71 cells in healthy and HCC patients was significantly higher than that in the control group (increased 1-49 to 3-48 times), and the killing activity of HepG2/hB71 cells was E/. When T=5:1, they are 57-2 and 43-8, respectively, with significant difference (P<0-05). When E/T=10:1 and 20:1, they reach 82-8 to 84-, respectively. 4 and 78-6 to 86-4, the difference was not significant (P > 0-05). Conclusion: B71 molecule significantly enhances the killing activity of NK cells on hepatoma cells in vitro, and may play a role in anti-hepatocarcinoma recurrence by activating non-specific cellular immunity.