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目的:分析H7N9流感病毒的HA、NA蛋白的抗原表位;预测H7N9流感病毒相关的HLA-Ⅱ类等位基因及易感人群。方法:软件分析HA、NA的同源性、B细胞表位、T细胞表位以及与HA、NA结合力强的HLA-Ⅱ类等位基因;并根据该等位基因在亚洲不同地域的基因频率,预测H7N9的易感人群。结果:H7N9流感病毒株之间氨基酸序列相对保守;HA具有10个B细胞表位和15个T细胞表位;NA有12个B细胞表位和9个T细胞表位;HLA等位基因DRB1*0701与HA、NA具有强结合力,在新疆、哈尔滨、山东、辽宁、北京、石家庄、天津等多个中国北方地区人群中的基因频率较高,高于广东、云南、台湾地区、日本和韩国。结论:预测了HA、NA的抗原性表位,为H7N9流感病毒的疫苗研究提供了理论基础;HLA-DRB1*0701与H7N9流感病毒高度相关;H7N9流感病毒在新疆、哈尔滨、山东、辽宁、北京、石家庄、天津地区更易传播。
Objective: To analyze the antigenic epitopes of HA and NA proteins of H7N9 influenza virus and to predict HLA-Ⅱ alleles and susceptible populations associated with H7N9 influenza virus. Methods: The software was used to analyze the homology of HA, NA, B cell epitopes, T cell epitopes and the HLA-Ⅱ alleles with strong binding to HA and NA. According to the genes of different alleles in Asia Frequency, Predict H7N9 in Susceptible Population. Results: The amino acid sequence of H7N9 influenza virus strains was relatively conservative. HA had 10 B cell epitopes and 15 T cell epitopes. NA had 12 B cell epitopes and 9 T cell epitopes. HLA allele DRB1 * 0701 has a strong binding force with HA and NA, and has a higher frequency in many northern China such as Xinjiang, Harbin, Shandong, Liaoning, Beijing, Shijiazhuang and Tianjin and higher than that of Guangdong, Yunnan, Taiwan, Japan and Korea. Conclusion: The antigenic epitopes of HA and NA were predicted, which provided a theoretical basis for the vaccine research of H7N9 influenza virus. HLA-DRB1 * 0701 was highly correlated with H7N9 influenza virus. H7N9 influenza virus was highly expressed in Xinjiang, Harbin, Shandong, Liaoning and Beijing Shijiazhuang, Tianjin area more easily spread.