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miR1444a是杨树特异的miR1444家族中的一员,在植物对干旱、机械和铜等环境胁迫的响应中发挥重要作用.本研究通过RACE技术克隆得到毛果杨MIR1444a基因.该基因全长691bp,含单一外显子.生物信息学分析发现其启动子区存在大量胁迫响应相关的顺式作用元件,其中以前报道的5个与铜胁迫相关的CuRE元件中有4个位于转录本的5′末端,1个在转录起始点上游.qRT-PCR分析miR1444a成熟体及其靶基因PtPPO3和PtPPO6的表达与锌胁迫的关系,发现随着Zn2+浓度的增加,毛果杨根、茎、叶中miR1444a的表达均呈上升趋势,而PtPPO3和PtPPO6的表达均下降,说明miR1444a受Zn2+调控,参与毛果杨对锌胁迫的响应.
miR1444a is a member of the poplar-specific miR1444 family and plays an important role in plant response to environmental stresses such as drought, mechanical and copper.In this study, the MIR1444a gene was cloned by RACE, which was 691 bp in length, Containing a single exon.Bioinformatics analysis found that there are a large number of stress response-related cis-acting elements in its promoter region, of which four previously reported CuRE elements related to copper stress are located at the 5 ’end of the transcript , One upstream of the transcriptional start point.Relationship between the expression of miR1444a and its target genes PtPPO3 and PtPPO6 and zinc stress was analyzed by qRT-PCR and found that with the increase of Zn2 + concentration, miR1444a The expression of PtPPO3 and PtPPO6 decreased, which indicated that miR1444a was regulated by Zn2 + and involved in the response of Populus tomentosa to zinc stress.