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用肿瘤细胞免疫山羊，取脾经匀浆透析法制备抗肿瘤特异性转移因子（Ｓ～ＴＦ），并观察了Ｓ～ＴＦ在体内外对淋巴因子激活的杀伤细胞（ＬＡＫ）细胞活性及ＬＡＫ细胞特异细胞毒作用及对荷瘤小鼠生存期的影响。结果显示，Ｓ－ＴＦ在体内外均能显著增加荷瘤宿主的ＬＡＫ活性及ＬＡＫ特异细胞毒作用，早期单独应用可明显延长荷瘤小鼠生存期，与ＬＡＫ／ＩＬ－２联合应用可明显地延长荷瘤小鼠生存期，并可治愈部分荷瘤小鼠（３／１０），其治疗效果明显优于ＬＡＫ／ＩＬ－２，原因可能是Ｓ－ＴＦ能诱导ＬＡＫ细胞为ＴＩＬ样细胞，明显增加ＬＡＫ细胞对肿瘤细胞的杀伤作用。提示Ｓ－ＴＦ能有效地提高ＬＡＫ／ＩＬ－２过继治疗效果。
The goats were immunized with tumor cells and anti-tumor specific transfer factor (S-TF) was prepared by dialysis with spleen homogenate. The activity of LAK cells and lymphokine-activated killer cells (LAK) cells in vitro and in vivo were observed. Specific cytotoxicity and its effect on the survival of tumor-bearing mice. The results showed that S-TF can significantly increase the LAK activity and LAK specific cytotoxicity of the tumor-bearing host both in vitro and in vivo. Early use alone can significantly prolong the survival of tumor-bearing mice, and the combined use with LAK/IL-2 can be significantly improved. Extending the survival time of tumor-bearing mice, and curing some tumor-bearing mice (3/10), the therapeutic effect is significantly better than LAK/IL-2, possibly because S-TF can induce LAK cells to be TIL-like cells. Increase the killing effect of LAK cells on tumor cells. It is suggested that S-TF can effectively enhance the effect of adoptive treatment of LAK/IL-2.