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采用浓度为10μL/L的外源乙烯处理未熟和完熟网纹甜瓜(20℃、24h)研究外源乙烯对果实乙烯生物合成的影响。结果表明:无论果实是否达到完熟状态,外源乙烯处理使未熟和完熟网纹甜瓜果实的硬度迅速降低,但是不同成熟度的甜瓜果实对外源乙烯的敏感性存在差异,即与未成熟的果实相比,外源乙烯使成熟果实硬度下降的速度更快,果实中ACC含量、ACC合成酶和ACC氧化酶活性更高,从而使成熟果实的内源乙烯释放量显著高于未成熟果实;从对外源乙烯的响应时间来看,乙烯处理后的成熟果实中ACC含量、ACC合成酶和ACC氧化酶活性立即升高。而外源乙烯处理后的1~6d,未成熟果实中ACC含量、ACC合成酶和ACC氧化酶活性与对照果实差异不显著,但是随着贮藏时间的进一步延长,乙烯处理与对照差异水平逐渐加大,说明外源乙烯的处理未成熟果实存在滞后期。
The effects of exogenous ethylene on the ethylene biosynthesis in fruit were studied by using exogenous ethylene at a concentration of 10 μL / L to treat unripe and mature reticulated muskmelon (20 ℃, 24h). The results showed that the hardness of immature and mature reticulated melon fruits decreased rapidly with exogenous ethylene treatment, regardless of whether the fruit matured or not. However, the sensitivities of melon fruits with different maturity to exogenous ethylene were different, that is, with the unripe fruit phase Compared with the control, exogenous ethylene decreased the hardness of mature fruit more rapidly, and the ACC content, ACC synthase and ACC oxidase activity in fruit were higher, which made the endogenous ethylene release of mature fruit significantly higher than that of immature fruit; From the response time of the source ethylene, ACC content, ACC synthase and ACC oxidase activity in mature fruits after ethylene treatment immediately increased. However, the ACC content, ACC synthase activity and ACC oxidase activity in immature fruits were not significantly different from the control fruits after 1 ~ 6 days of exogenous ethylene treatment, but gradually increased with the prolongation of storage time Large, indicating that the treatment of exogenous ethylene immature fruit there is a lag period.