用细胞培养方法，在原代培养的大鼠皮质神经细胞上，观察了（－）Ｓ·Ｒ蝙蝠葛苏林碱对谷氨酸引起的神经元损伤的保护作用。以Ｆｕｒａ２／ＡＭ为Ｃａ２＋的荧光指示剂，用ＡＲＣＭＭＩＣ阳离子测定系统观察（－）Ｓ·Ｒ蝙蝠葛苏林碱对谷氨酸诱发大鼠脑皮质神经细胞内Ｃａ２＋升高的影响。结果表明（－）Ｓ·Ｒ蝙蝠葛苏林碱能剂量依赖性地抑制谷氨酸的神经毒作用，对谷氨酸诱发的神经细胞内游离Ｃａ２＋升高有明显的抑制作用。提示蝙蝠葛苏林碱对缺血性脑损伤有保护作用。 Cell culture was used to observe the neuroprotective effect of (-) S · Rdryingosine on glutamate-induced neuronal damage in primary cultured rat cortical neurons. Using Fura2 / AM as fluorescent indicator of Ca2 +, ARCMMIC cationic assay was used to observe the effect of (-) S · Rdryingosine on glutamate-induced intracellular Ca2 + in cortical neurons of rats influences. The results showed that (-) S · Rdryingosine could inhibit the neurotoxic effect of glutamate in a dosedependent manner, and significantly inhibit the increase of free Ca2 + in glutamate-induced neurons. Tip dauricine basement has a protective effect on ischemic brain injury.