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目的观察经γ射线照射后不同时相点小鼠骨髓嗜多染红细胞微核率的变化,进一步完善微核流式细胞仪自动化检测方法。方法 ICR雄性小鼠给予60Coγ射线一次性全身照射,每组4只,剂量分别为1.0、3.0和6.0 Gy,与照射后0.5、2、6和12 h取材;阴性对照组给予假照射;阳性对照组注射环磷酰胺24 h后取材。结果 1.0 Gyγ射线照射后6 h,骨髓f MNPCE显著升高(P<0.01);而3.0 Gy及6.0 Gy照射后6 h,骨髓f MNPCE显著升高(P<0.01);2种方法检测结果显示均有较好的剂量、时间依赖性,并呈显著正相关性(r=0.962,P<0.05)。结论红细胞微核流式细胞仪自动化检测方法快速、简单、灵敏、客观,完全适用于小鼠骨髓红细胞微核率的检测。
Objective To observe the change of micronucleus rate of bone marrow polychromatic erythrocytes in mice at different time points after γ-ray irradiation, and to further improve the automated detection method of micronucleus flow cytometry. Methods ICR male mice were given a one-time whole-body irradiation with 60Coγ-ray, with 4 doses of 1.0, 3.0 and 6.0 Gy respectively. The irradiated mice were exposed at 0.5, 2, 6 and 12 h after irradiation. The negative control group was given sham irradiation. Group injection of cyclophosphamide 24 h after drawing. Results The f MNPCE of bone marrow increased significantly (P <0.01) 6 h after irradiation with 1.0 Gy γ-ray, whereas f MNPCE of bone marrow increased significantly (P <0.01) 6 h after 3.0 Gy and 6.0 Gy irradiation. The results of two methods Both had better dose and time dependence, and showed a significant positive correlation (r = 0.962, P <0.05). Conclusion The automated detection method of erythrocyte micronucleus flow cytometry is rapid, simple, sensitive and objective and can be completely applied to the detection of micronuclei in mouse bone marrow erythrocytes.