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对“晋麦8号”小麦幼苗分别采用5 mW·mm~(-2)He-Ne激光辐照,10.08 kJ·m~(-2)·d~(-1)增强UV-B辐射及二者组合处理。经研究发现:增强UV-B辐射可引起小麦叶片类囊体膜光吸收能力减弱,偶联因子(ATPase)(Mg~(2+)-ATPase和Ca~(2+)-ATPase)活性降低和光合磷酸化(环式和非环式)活性抑制,说明增强UV-B辐射引起小麦类囊体膜损伤。而一定剂量的He-Ne激光辐照可部分修复增强UV-B对小麦类囊体膜的损伤,使叶片类囊体膜光吸收能力升高,ATPase(Mg~(2+)-ATPase和Ca~(2+)-ATPase)活性升高,光合磷酸化(环式和非环式)活性受到激活。SDS-PAGE结果表明,各处理组类囊体膜多肽成分与对照没有明显变化,增强UV-B辐射可导致多肽含量减少,而He-Ne激光辐照可使多肽含量有所增加。
UV-B radiation was increased by 10.08 kJ · m -2 (-2) d -1 with 5 mW · mm -2 He-Ne laser irradiation on “Jinmai No.8” wheat seedlings. And the combination of the two. The results showed that enhanced UV-B radiation could reduce the light absorption ability of wheat leaf thylakoid membrane, reduce the activity of ATPase (Mg ~ (2 +) - ATPase and Ca ~ (2 +) - ATPase) Photosynthetic phosphorylation (both cyclic and acyclic) activity inhibition, indicating enhanced UV-B radiation caused wheat thylakoid membrane damage. However, irradiation with a certain dose of He-Ne laser could partly repair the damage of thylakoid membrane of wheat by enhanced UV-B, and increase the light absorption capacity of thylakoid membrane. The ATPase (Mg ~ (2 +) - ATPase and Ca ~ (2 +) - ATPase) activity is increased, and photosynthetic phosphorylation (both cyclic and acyclic) activity is activated. The result of SDS-PAGE showed that there was no significant change in the composition of thylakoid membranes between the treated groups and the control group. Increasing the UV-B radiation could lead to the decrease of the polypeptide content, but He-Ne laser irradiation could increase the polypeptide content.