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目的:观察黄芩苷对脂质过氧化及内皮细胞氧化损伤的保护作用。方法:50只小鼠分为正常对照组及模型组、黄芩苷低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量组,每组10只,采用高脂饮食联合维生素D3建立AS模型,给药组在建立模型同时给药4 w。检测血脂水平、血清超氧化物歧化酶(SOD)、丙二醛(MDA)和一氧化氮(NO)水平。另采用H2O2体外诱导人脐静脉内皮细胞损伤的方法,建立人脐静脉内皮细胞损伤模型,四甲基偶氮唑蓝(MTT)法检测不同浓度黄芩苷对ECV304细胞增殖的影响。结果:黄芩苷高剂量组血清CHO、TG、LDL-C、MDA水平较模型组显著降低(P<0.05或P<0.01),HDL-C、SOD和NO水平显著升高(P<0.05或P<0.01),不同质量浓度的黄芩苷可以剂量依赖地减少H2O2诱导的细胞凋亡,恢复血管内皮细胞增殖。结论:黄芩苷对调节脂质代谢、抗脂质过氧化,减轻血管内皮细胞的氧化损伤,促血管新生有良好的作用。
Objective: To observe the protective effects of baicalin on lipid peroxidation and endothelial cell oxidative damage. Methods: Fifty mice were divided into normal control group and model group. Baicalin (50 mg / kg), middle dose (100 mg / kg), high dose (200 mg / kg) Fat diet combined with vitamin D3 to establish AS model, the administration group in the establishment of the model while given 4 w. Blood lipid levels, serum superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) levels were measured. In addition, H2O2 was used to induce human umbilical vein endothelial cell injury in vitro. Human umbilical vein endothelial cell injury model was established. The effects of different concentrations of baicalin on the proliferation of ECV304 cells were detected by MTT assay. Results: The levels of serum CHO, TG, LDL-C and MDA in high-dose baicalin group were significantly lower than those in model group (P <0.05 or P <0.01), and the levels of HDL-C, <0.01), different concentrations of baicalin can dose-dependently reduce H2O2-induced apoptosis and restore vascular endothelial cell proliferation. Conclusion: Baicalin plays a good role in regulating lipid metabolism, anti-lipid peroxidation, reducing oxidative damage of endothelial cells and promoting angiogenesis.