论文部分内容阅读
本研究以高抗大白菜芝麻状斑点病品系‘C24’和高感品系‘03B9’为试验材料,利用cDNA-AFLP技术分析其在发病条件(高氮)和不发病或少发病条件(低氮)下基因表达的差异,并回收得到与大白菜芝麻状斑点病相关的差异表达转录衍生片段(Transcript-derived fragment,TDF)19条。研究结果表明:12个TDF与NCBI或Brassica Database已有序列同源,功能涉及激素调节、氧化还原酶、细胞结构、蛋白质分解、信号传导等;3个TDF在NCBI或TIGR上有序列同源,但是功能未知;4个TDF未找到同源序列。结合本课题组有关氮素浓度和形态对大白菜芝麻状斑点病影响的生理指标的研究结果,可推测大白菜芝麻状斑点病可能是细胞结构受到破坏,细胞膜上细胞信号跨膜转导受阻,ACC、ABA的调节,蛋白质的降解,PPO引起酚类物质褐变等共同作用所致。
In this study, the high resistance to cabbage sesame speckle disease line ’C24’ and the high susceptible line ’03B9’ were used as materials to analyze the relationship between the disease conditions (high nitrogen) and non-disease or less incidence (low nitrogen ), And 19 differentially expressed Transcript-derived fragments (TDFs) related to sesame blight of Chinese cabbage were recovered. The results showed that 12 TDFs had sequence homology with NCBI or Brassica Database, and their functions involved in hormonal regulation, oxidoreductase, cell structure, proteolysis and signal transduction. Three TDFs had sequence homology on NCBI or TIGR, But the function is unknown; 4 TDFs did not find homologous sequences. Combined with our research group on the physiological and biochemical indicators of the effects of nitrogen concentration and morphology on the sesame-shaped speckle disease in Chinese cabbage, it can be inferred that the senescence-induced speckle disease of Chinese cabbage may result in the disruption of the cell structure, the obstruction of the transmembrane signal transduction of the cell signal on the cell membrane, ACC, ABA regulation, protein degradation, PPO caused by the phenolic browning and other common effects.