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本实验用腹腔注射链佐霉素(Streptozotocin简称STZ)方法破坏小鼠胰岛B细胞以诱发高血糖,观察生长抑素(Somatostatin,SS)、神经降压素(neurotensin,NT)、胰高血糖素(glucagon,GC)和促甲状腺素释放激素(TRH)4种胃肠激素对小鼠高血糖的影响。在每天腹腔注射STZ(60mg/kg)前10分钟分别经皮下注射上述4种胃肠激素,连续注射5天,在实验的第1,6,8,10,15天取血测血清葡萄糖浓度,对照组注射生理盐水(NS)。结果发现:(1)预先注射SS和NT可不同程度地抑制由STZ引起的实验性高血糖,并呈剂量一效应关系,(2)预先注射GC和TRH,血糖浓度仍明显升高,与NS对照组比较无显著差异,(3)取注射STZ后第15天的高血糖小鼠(血糖高于350mg％者)分为8组,分别以SS和NT作治疗性注射,每天一次共5日,并未见对小鼠高血糖有缓解效果;(4)正常小鼠单独皮下注射NS、SS、NT、GC、和TRH,每天一次连续5天,在注射后15天内未见对血糖水平有明显影响。以上结果提示:预防性注射SS和NT可显著抑制由STZ诱发的高血糖的产生。
In this experiment, intraperitoneal injection of streptozotocin (STZ) method was used to destroy mouse islet B cells to induce hyperglycemia. Somatostatin (SS), neurotensin (NT) and glucagon were observed. Effects of glucagon (GC) and thyrotropin-releasing hormone (TRH) on hyperglycemia in mice. The above four gastrointestinal hormones were injected subcutaneously 10 minutes before the daily intraperitoneal injection of STZ (60 mg/kg). The blood glucose concentration was measured on days 1, 6, 8, 10, and 15 of the experiment. The control group was injected with saline (NS). The results were as follows: (1) Pre-injection of SS and NT inhibited the experimental hyperglycemia caused by STZ in different degrees, and showed a dose-effect relationship. (2) Pre-injection of GC and TRH, blood glucose concentration was still significantly increased, and NS There was no significant difference between the control group. (3) The hyperglycemic mice (blood glucose higher than 350 mg%) on the 15th day after the injection of STZ were divided into 8 groups and injected with SS and NT as therapeutic injections, once a day for 5 days. , No effect of alleviating hyperglycemia in mice; (4) Normal mice were injected subcutaneously with NS, SS, NT, GC, and TRH, once a day for 5 consecutive days, and no blood sugar levels were seen within 15 days after injection. Obvious effect. The above results suggest that prophylactic injection of SS and NT can significantly inhibit the production of hyperglycemia induced by STZ.