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目的探讨清道夫受体BⅠ(SR-BⅠ)在动脉粥样硬化发生中的作用及阿托伐他汀抗动脉粥样硬化的作用机制。方法随机选择健康体检中心男性高TC血症患者60例为高脂组,另选男性TC正常者60例为对照组。采用紫外分光法检测血管紧张素转换酶(ACE)的活性,放射免疫法检测血管紧张素Ⅱ(AngⅡ)水平,硝酸还原酶法检测NO水平,RT-PCR和Western blot法分别检测SR-BⅠ mRNA和蛋白表达水平。结果与对照组比较,高脂组TC、TG、LDL-C、AngⅡ、ACE明显升高(P<0.05,P<0.01),HDL-C、NO、SR-BⅠ mRNA和蛋白表达明显降低(P<0.05,P<0.01);与同组治疗前比较,高脂组治疗后TC、TG、LDL-C、Ang Ⅱ、ACE明显降低(P<0.05),HDL-C、NO、SR-BⅠ mRNA和蛋白表达明显升高(P<0.05,P<0.01)。HDL-C与SR-BⅠ呈显著正相关,AngⅡ与SR-BⅠ呈显著负相关。结论高TC血症患者SR-BⅠ表达降低,阿托伐他汀能上调SR-BⅠ表达。
Objective To investigate the role of scavenger receptor B Ⅰ (SR-B Ⅰ) in the pathogenesis of atherosclerosis and the mechanism of atorvastatin against atherosclerosis. Methods Sixty patients with hypercholesteremia were selected randomly as hyperlipidemia group and 60 normal male TC as male control group. The activity of angiotensin converting enzyme (ACE) was detected by ultraviolet spectrophotometry, the level of angiotensin Ⅱ (AngⅡ) was measured by radioimmunoassay, the level of NO was detected by nitrate reductase method, and the expression of SR-BⅠ mRNA was detected by RT-PCR and Western blot respectively And protein expression level. Results Compared with the control group, the levels of TC, TG, LDL-C, AngⅡ and ACE in the hyperlipidemia group were significantly increased (P <0.05, P <0.01) (P <0.05, P <0.01). Compared with the same group before treatment, the levels of TC, TG, LDL-C, Ang Ⅱ and ACE were significantly decreased And protein expression was significantly increased (P <0.05, P <0.01). There was a significant positive correlation between HDL-C and SR-BⅠ, while there was a significant negative correlation between AngⅡ and SR-BⅠ. Conclusions The expression of SR-B Ⅰ is decreased in patients with hypercholesteremia and atorvastatin can up-regulate the expression of SR-B Ⅰ.