在强碱性水溶液中(pH>12),钙黄绿素与Ca~(2+)的络合物具有荧光性质,而钙黄绿素本身在此条件下不产生荧光。根据这一性质,陕西师范大学化学系吕九如等老师在自制的多功能光导纤维光度计上建立了血清中钙的荧光滴定法,本方法以钙黄绿素为荧光指示剂,用EDTA标准溶液对强碱性试样溶液进行滴定。根据滴定过程中试液荧光强度与滴加EDTA标准溶液体积的关系曲线一滴 In strongly basic aqueous solutions (pH> 12), the complex of calcein and Ca 2+ fluoresces, whereas calcein itself does not fluoresce under these conditions. According to this nature, Shaanxi Normal University, Department of Chemistry, such as Lv Jiu-ru and other teachers in the homemade multi-functional optical fiber photometer to establish a serum calcium fluorescence titration method, the method of calcein as a fluorescent indicator, with EDTA standard solution of alkali The sample solution is titrated. According to the titration of the fluorescence intensity of the test solution and the dropping of EDTA standard solution volume curve of a drop