论文部分内容阅读
为探讨力竭运动后恢复24 h肾脏NO含量变化特点与肾损伤程度关系,将24只SD大鼠随机分为安静对照组(C)、低强度力竭组(LE)和高强度力竭组(HE)。一次性跑台力竭运动后24 h取材,检测肾组织NO含量、NOS活性和血清尿素氮、血清肌酐、尿蛋白含量,透射电镜观察肾细胞凋亡等病理变化。结果表明:HE组的肾组织NO含量和NOS活性明显低于LE组(P<0.05~0.01);HE组的血清尿素氮、血清肌酐和尿蛋白含量明显异常(P<0.05~0.01),LE组仅血清尿素氮浓度明显异常(P<0.05);透射电镜下两运动组的肾小管上皮细胞线粒体损害,且HE组的肾实质细胞凋亡显著增加(P<0.05)和偶见坏死。结论:强度不同的力竭运动恢复24 h,大鼠肾组织的NO含量变化趋势不同,高强度力竭运动后肾实质细胞损伤、肾功能恢复缓慢与肾组织的NO含量水平降低有关。
In order to explore the relationship between the changes of kidney NO content and the degree of renal injury in 24 h after exhaustive exercise, 24 SD rats were randomly divided into 4 groups: control group (C), low intensity exhaustion group (LE) and high intensity exhaustion group (HE). Twenty-four hours after exhaustive treadmill exercise, the contents of NO, NOS, serum urea nitrogen, serum creatinine and proteinuria in renal tissue were measured. The pathological changes of renal cell apoptosis were observed by transmission electron microscopy. The results showed that the content of NO and the activity of NOS in renal tissue of HE group were significantly lower than that of LE group (P <0.05-0.01). The contents of serum urea nitrogen, serum creatinine and urine protein in HE group were significantly abnormal (P <0.05-0.01) (P <0.05). The mitochondrial damage of tubular epithelial cells in the two exercise groups under transmission electron microscope and the apoptosis of renal parenchymal cells in HE group were significantly increased (P <0.05) and occasionally necrotic. CONCLUSION: After 24 h of exhaustive exercise with different intensities, the changes of NO content in rat kidney tissue are different. Injury of renal parenchymal cells and slow recovery of renal function after high intensity exhaustive exercise are related to the decrease of NO content in renal tissue.