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目的建立高效液相法(HPLC)测定四季三黄丸中黄芩苷和盐酸小檗碱的含量。方法采用C18柱,以甲醇-乙腈-0.1%磷酸溶液(用三乙胺调节pH3.0±0.1)=23:23:54为流动相,检测波长274nm,流速1.0ml/min。结果黄芩苷在0.64～3.25μg范围内线性关系良好,R=0.9999;盐酸小檗碱在0.32～1.7μg范围内线性关系良好,R=0.9995。结论本方法操作简便、方法可靠、结果准确、重现性好,可作为该产品质量控制的方法。
Objective To establish HPLC method for the determination of baicalin and berberine hydrochloride in Shiji Sanhuang Wan. Methods The mobile phase consisted of methanol - acetonitrile - 0.1% phosphoric acid solution (adjusted to pH3.0 ± 0.1 with triethylamine) = 23:23:54 by C18 column. The detection wavelength was 274 nm and the flow rate was 1.0 ml / min. Results Baicalin showed good linearity in the range of 0.64-3.25μg, R = 0.9999. Berberine hydrochloride showed good linearity in the range of 0.32-1.7 μg with R = 0.9995. Conclusion The method is simple, reliable, accurate and reproducible. It can be used as a method for quality control of this product.