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我们应用磷脂、胆固醇等原料，通过溶溶法制成５－氟尿嘧啶（５－Ｆｕ）多相脂质体，包封率为５２．５％，脂质体粒径小于２μｍ者占９８％，对冷热均有较好的稳定。然后将８只健康成年新西兰长耳兔分为４组，每组２只。第一组不用药物，作为对照。其余各组用５－Ｆｕ多相脂质体０．２ｍｌ（浓度为２５ｍｇ／ｍｌ）注射于兔眼球结膜下，每隔３日注射１次，共注射４次。分别距最后一次注射后１２小时、７日和１４日将２、３和４组兔眼注射点相对应处睫状体完整取出。对照组兔眼相同部位的睫状体与第４组同日取出，制成电镜标本行透射电镜检查。结果发现：４组兔眼睫状上皮细胞的超微结构无任何改变。实验表明每隔３天结膜下注射５ｍｇ５－Ｆｕ多相脂质体，共４次，对兔眼睫状体上皮细胞无明显毒性。
We use phospholipid, cholesterol and other raw materials, made by dissolving 5-fluorouracil (5-Fu) multiphase liposomes, entrapment efficiency was 52.5%, liposome size less than 2μm accounted for 98% of the hot and cold All have better stability. Eight healthy adult New Zealand long-eared rabbits were then divided into four groups of two. The first group did not use drugs as a control. The remaining groups were injected with 0.2 ml of 5-Fu multi-phase liposome (concentration 25mg / ml) into the eyeball subconjunctival in rabbits every three days for a total of 4 times. Respectively from the last injection of 12 hours, 7 and 14, 2,3 and 4 groups of rabbit eye injection point at the corresponding ciliary body removed. The ciliary body in the same part of the rabbit eyes of the control group was taken out on the same day with the fourth group and made into a TEM for transmission electron microscopy. The results showed that there was no change in the ultrastructure of ciliary epithelium in 4 groups. Experiments show that every 3 days subconjunctival injection of 5mg5-Fu multi-phase liposomes, a total of 4 times, no significant toxicity of rabbit ciliary body epithelial cells.