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利用超支化的聚(3-乙基-3-羟甲基氧杂环丁烷)(HBPO)与1,3-丙烷磺内酯反应,制备了磺酸基修饰的水溶性的类肝素超支化聚醚(HBPO-SO3),1H-NMR和FTIR表征产物具有预期的结构.通过粒径分析仪、透射电镜(TEM)和荧光探针对聚合物在水溶液中的自组装行为进行表征,证明当HBPO-SO3在水溶液中的浓度大于0.017mg/mL时,可形成尺寸在25～70nm之间的具有疏水内核的胶束.复钙化凝血时间(PRT)的测定证明这种类肝素超支化聚醚具有良好的抗凝血性能,体外细胞活性测定和细胞形貌观察证明其具有良好的细胞相容性.
A sulfonic acid group-modified water-soluble heparinoid hyperbranched was prepared by the reaction of hyperbranched poly (3-ethyl-3-hydroxymethyloxetane) (HBPO) with 1,3-propane sultone The structures of HBPO-SO3, 1H-NMR and FTIR were characterized by means of particle size analyzer, transmission electron microscopy (TEM) and fluorescence probe. The self-assembly behavior of polymer in aqueous solution was characterized by The concentration of HBPO-SO3 in aqueous solution above 0.017 mg / mL results in the formation of micelles with hydrophobic core sizes between 25 and 70 nm.The determination of the recalcification clotting time (PRT) demonstrates that this heparinoid hyperbranched polyether has Good anticoagulant properties, in vitro cell viability assay and cell morphology observation showed that it has good cell compatibility.