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以小麦晋2148,7506,7606,5926 4个春性品种的幼胚为材料,用JQ700型高速基因枪将PBI121质粒导入小麦,研究并确立了轰击速度、钨粉直径、上样方式、DNA浓度等基因枪转化参数。在以上研究基础上进一步将导入外源基因的小麦幼胚质片细胞通过胚胎发生途径再生成植株。供试的4个品种中有3个(7506,7606,5926)获得了可育的转基因植株。GUS基因的转化频率分别为0.6％,0.6％和1.4％。Southern杂交表明GUS基因(β-葡糖苷酸酶基因,β-Glucuronidase)已整合到小麦的基因组中,并可传递至R1代。R1代G418抗性测试表明,NPTⅡ基因(新酶素磷酸转移酶基因)的传递符合Mendel单基因显性遗传规律。
The wheat embryo embryos of 4 spring wheat varieties Jinjin 2148,7506,7606,5926 were used as material to introduce PBI121 plasmid into wheat with JQ700 high-speed gene gun. The effects of bombardment speed, tungsten powder diameter, loading mode, DNA concentration Other gene gun conversion parameters. On the basis of the above studies, we further regenerated the wheat embryo-derived sheet cells from the foreign gene into plants through the embryogenesis pathway. Three of the four cultivars tested (7506, 7606, 5926) obtained fertile transgenic plants. The frequency of GUS gene transformation was 0.6%, 0.6% and 1.4%, respectively. Southern hybridization indicated that the GUS gene (β-glucuronidase gene, β-Glucuronidase) has been integrated into the genome of wheat and passed to the R1 generation. The R1 generation G418 resistance test showed that the NPTⅡ gene (new enzyme phosphotransferase gene) transmission in line with Mendel single-gene dominant inheritance.