论文部分内容阅读
目的:优选出节节草总黄酮最佳提取工艺及测定总黄酮抗氧化活性。方法:以总黄酮含量为指标采用正交设计的方法研究总黄酮提取工艺,以DPPH法测定总黄酮抗氧化活性。结果:最佳提取工艺为加10倍量70%乙醇,加热回流提取3次,每次1.5h。抗氧化实验表明当总黄酮含量52.80%时,对DPPH自由基的清除率达到82.98%。结论:节节草总黄酮提取工艺稳定、可靠,且体外有明显抗氧化活性。
Objective: To optimize the optimal extraction process of total flavonoids of Artragah and determine the antioxidant activity of total flavonoids. Methods: Total flavonoids were used as indexes to study the extraction technology of total flavonoids by orthogonal design. The antioxidant activity of total flavonoids was determined by DPPH method. Results: The best extraction process was adding 10 times the amount of 70% ethanol, heating and refluxing extraction three times, each time 1.5h. Antioxidant experiments showed that when the total flavonoids content of 52.80%, DPPH radical scavenging rate reached 82.98%. Conclusion: The extraction of total flavonoids from Artragah sagitti is stable and reliable, and has obvious antioxidant activity in vitro.