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In this report,complete mitochondrial genome sequences of Laminaria cultivation variety ‘Rongfu were obtained.The results showed the length of circular molecule of mtDNA was 37638 bp (64.7％ A+T),encoding three rRNAs (23S,16S and 5S),25tRNAs,35 known mitochondrial proteins and 3ORFs.Sequence alignment indicated its mtDNA genome was very similar to that of Laminaria japonica.Phylogenetic trees inferred from concatenated 30 mitochondrial genes showed that ‘Rongfu,Laminariajaponica,Laminaria longipedalis,Laminaria diabolica,Laminaria religiosa and Laminaria ochotensis clustered together.In addition,compared with mitochondrial genome of L.japonica,‘Rongfu mtDNA lacked a non-coding region of 19 nucleotides,which was located between rRNA small subunit gene 3 (rps3) and rRNA small subunit gene 9 (rps9).Seven cultivation varieties of China were divided into two groups based on this non-coding region which was absent in ‘Rongfu,‘Fujian and ‘Sanhai while present in ‘Ailunwan,‘Dongfang No.2,‘Dongfang No.3 and‘Zaohoucheng.So this variation can be used in germplasm identification of cultivation variety.