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目的:探讨有氧运动对高蛋氨酸饲料饮食大鼠血浆同型半胱氨酸(Hcy)水平和抗氧化应激能力的影响,为运动预防高半胱氨酸血症(HH)和动脉粥样硬化(AS)发生提供实验参考。方法:雄性健康Wistar大鼠24只,随机分为高蛋氨酸饲料饮食组(M组)、高蛋氨酸饲料饮食+有氧运动组(M+T组)和正常安静对照组(C组);M组和M+T组给予含3%蛋氨酸的高蛋氨酸饲料,C组喂普通颗粒饲料,M+T组同时每日进行90分钟无负重游泳运动。8周实验结束后,检测各组大鼠血浆Hcy水平、血浆超氧化物歧化酶(SOD)活性、脂质过氧化物(MDA)及还原型谷胱甘肽(GSH)含量。结果:与C组相比:M组血浆Hcy水平显著升高(P<0.01),MDA含量、SOD活性显著升高(P<0.05),GSH含量无显著改变(P>0.05);M+T组血浆Hcy水平轻微升高,MDA含量略有增加,但无显著性差异(P>0.05),SOD活性和GSH含量显著升高(P<0.05)。与M组相比,M+T组血浆Hcy水平显著下降(P<0.01),GSH含量显著升高(P<0.05),MDA含量及SOD活性略有下降,但无显著性差异(P>0.05)。结论:高蛋氨酸饲料饮食可诱导大鼠HH形成和体内高氧化应激状态,适宜的有氧运动可以预防蛋氨酸诱导HH的形成,增强机体抗氧化应激能力,从而有助于预防血管内皮损伤和AS的形成。
Objective: To investigate the effects of aerobic exercise on plasma homocysteine (Hcy) level and antioxidant capacity in rats fed high methionine diet, and to investigate the effects of exercise on preventing hyperhomocysteinemia (Hcy) and atherosclerosis (AS) occurs to provide experimental reference. Methods: Twenty-four male Wistar rats were randomly divided into high methionine diet group (M group), high methionine diet group (diet + aerobic exercise group) and normal quiet control group (C group) And M + T group were fed with high methionine diet containing 3% methionine. Group C was fed with normal pellet feed, and M + T group was given 90-day non-weight-bearing swimming exercise daily. After 8 weeks of experiment, plasma Hcy level, plasma SOD activity, lipid peroxidation (MDA) and reduced glutathione (GSH) were measured. Results: Compared with group C, plasma Hcy levels in M group were significantly increased (P <0.01), MDA content and SOD activity were significantly increased (P <0.05), GSH content was not significantly changed (P> 0.05) Plasma homocysteine level increased slightly and MDA content increased slightly, but there was no significant difference (P> 0.05). SOD activity and GSH content increased significantly (P <0.05). Compared with M group, plasma Hcy level in M + T group was significantly decreased (P <0.01), GSH content was significantly increased (P <0.05), MDA content and SOD activity decreased slightly, but there was no significant difference ). CONCLUSION: High-methionine diet can induce the formation of HH and hyper-oxidative stress in rats. Appropriate aerobic exercise can prevent the formation of HH by methionine and enhance the anti-oxidative stress ability of the body, thus contributing to the prevention of vascular endothelial injury and AS formation.