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目的探讨转染NF-κB诱捕物寡核苷酸(decoy ODN)对肝脏缺血再灌注损伤的影响。方法制作20只肝脏缺血再灌注SD大鼠模型,无序ODN对照组、decoy ODN实验组各10只。阻断肝脏血流后,实验组行脂质体包裹的NF-κBdecoy ODN溶液门静脉注射转染,对照组行脂质体包裹的无序ODN门静脉注射。开放肝脏血流后3h取出肝脏,应用病理学、EMSA和RT-PCR分别检测移植肝脏病理改变、肝脏组织NF-κB转录活性和TNF-αmRNA表达情况。结果实验组肝脏损伤减轻,肝细胞NF-κB转录活性、TNF-α mRNA表达较对照组明显减小或减少(均P<0.01)。结论 NF-κBdecoy ODN可有效抑制肝脏细胞NF-κB的激活从而抑制肝脏缺血再灌注损伤。
Objective To investigate the effect of transfection of decoy ODN on hepatic ischemia-reperfusion injury. Methods Twenty SDI rat models of liver ischemia-reperfusion were made, and 10 rats were randomly assigned to ODN control group and decoy ODN group. After blocking the hepatic blood flow, the experimental group was injected with lipofuscin NF-κBdecoy ODN solution by portal vein, and the control group was injected with liposomal encapsulated ODN portal vein. The liver was removed 3h after the open hepatic blood flow, and pathological changes, EMSA and RT-PCR were used to detect liver pathological changes, liver NF-κB transcriptional activity and TNF-αmRNA expression. Results In the experimental group, the liver injury was alleviated, the NF-κB transcriptional activity and TNF-α mRNA expression in hepatocytes were significantly decreased or decreased (all P <0.01). Conclusion NF-κBdecoy ODN can effectively inhibit the activation of NF-κB in liver cells and thus inhibit hepatic ischemia-reperfusion injury.