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以OT型百合‘黄天霸’(‘Manissa’)×O型东方百合‘西伯利亚’(‘Siberia’),OT型百合‘罗宾娜’(‘Robina’)×O型东方百合‘索邦’(‘Sorbonne’)进行杂交,利用体外受精和胚胎挽救技术获得OTO远缘杂交新材料。细胞学观察表明,从‘黄天霸’ב西伯利亚’获得的F1杂种为非整倍体植物,其染色体数目变化为16~48,其后代的气孔尺寸存在多样性,不同植株的气孔大小不同,甚至同一叶片的气孔尺寸也不同;从‘罗宾娜’ב索邦’获得的F1杂种为三倍体植物,其染色体数目是2n=3x=36,其后代的三倍体气孔尺寸为(93.2±1.04)×(70.4±0.53)。在大多数OT×O的营养繁殖后代中,它们的有丝分裂是规则的,但是观察到一些异常现象,例如核不均等分裂,染色体桥,染色体落后;核萌发,核管侵入并缢裂,形成微核。这些在有丝分裂期间的异常现象可能是产生染色体数目和结构变异的机制之一。
In this study, we used ’Oliver’ ’Oliver’ ’Siberia’ ’,’ Robina ’× O Oriental lily’ Sorbonne ’ (’Sorbonne’) hybridization, the use of in vitro fertilization and embryo rescue techniques to obtain new OTO hybrid materials. Cytological observations showed that F1 hybrids obtained from ’Huangtianba’ × ’Siberia’ were aneuploid plants with chromosome number changes ranging from 16 to 48, and the stoma size of their offspring varied, with different plant stoma sizes , And even the same leaf has different stomatal dimensions. F1 hybrid obtained from ’Robina’ x ’Sorbonne’ is a triploid plant with chromosome number 2n = 3x = 36, and its offspring’s triploid pore size is (93.2 ± 1.04) × (70.4 ± 0.53). In most of OT x O vegetative progeny, their mitosis is regular but some abnormal phenomena are observed, such as nuclear heterogeneity, chromosomal bridges, chromosomal traits, nuclear germination, nuclear invades and chapped to form micro nuclear. These anomalies during mitosis may be one of the mechanisms that produce chromosome numbers and structural variations.