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使用培养老化成纤维细胞,就甘草甜素(G)与糖皮质激素对细胞增殖的作用进行了比较,并对G促进细胞增殖作用的机制进行了研究。材料与方法:细胞用取自人皮肤片的成纤维细胞TIG-103,培养方法:以含10％胎牛血清(FBS)的达尔伯克氏改良伊格尔培养液(DME),在37℃5％CO_2培养箱中培养细胞。细胞融合成单层细胞时按1:2或1:4分裂传代培养,确认丧失增殖能力的传代
The effects of glycyrrhizin (G) and glucocorticoid on cell proliferation were compared using cultured aging fibroblasts, and the mechanism of G promoting cell proliferation was studied. MATERIALS AND METHODS: TIG-103 fibroblasts derived from human skin pieces were cultured in Dulbecco’s modified Eagle’s medium (DME) supplemented with 10% fetal bovine serum (FBS) Cells were cultured in 5% CO 2 incubator. The cells were passaged into 1: 2 or 1: 4 split passages when confluent into monolayer cells, confirming the passage of passages that lost the ability to proliferate