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对采自浙江、湖北和安徽3省的蚕豆赤斑病样品进行了病原菌的分离和鉴定,采用菌丝生长速率法检测了引起赤斑病的2种病原菌——蚕豆葡萄孢Botrytis fabae和灰葡萄孢B.cinerea的抗药性发生情况,并在离体条件下通过抗药性诱导试验比较了二者的抗药性风险。结果共分离得到153个菌株,其中蚕豆葡萄孢122株(占79.7%),灰葡萄孢31株(占20.3%)。共检测到37株多菌灵高水平抗药性菌株(其中蚕豆葡萄孢9株)和42株异菌脲低水平抗药性菌株(其中蚕豆葡萄孢17株);嘧霉胺对153个菌株的EC50值在0.01～5.13μg/mL之间,平均EC50值为0.72±0.15μg/mL;表明蚕豆赤斑病菌对常见杀菌剂已表现出一定的抗药性,且灰葡萄孢的抗药性问题比蚕豆葡萄孢要严重得多。抗药性诱导试验进一步证实,灰葡萄孢的抗药性风险明显高于蚕豆葡萄孢。
The pathogen was isolated and identified from the brown spot of Vicia faba in Zhejiang, Hubei and Anhui provinces. Two pathogenic fungi causing Botrytis cinerea, Botrytis fabae and gray grape, were detected by mycelium growth rate method. Spores B.cinerea resistance situation, and in vitro conditions by resistance induction test to compare the risk of both. Results A total of 153 isolates were isolated, of which 122 strains of Vicia faba (79.7%) and 31 strains of Botrytis cinerea (20.3%). A total of 37 highly resistant strains of carbendazim (including 9 strains of Botrytis cinerea) and 42 low resistant isolates (including 17 strains of Vicia faba) were detected. EC50 The average value of EC50 was 0.72 ± 0.15μg / mL. The results indicated that R. solani showed some resistance to common fungicides, and the resistance of Botrytis cinerea was higher than that of broad bean grape Spores are much more serious. Resistance induction test further confirmed that Botrytacin resistance was significantly higher than Botrytis cinerea.