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目的:研究Dextran对蛋白药物的释放影响。方法:将模型蛋白BSA溶解于多糖溶液中,通过W/O乳液法静电纺丝制备缓释纤维。采用MicroBCA法测定该纤维体外释放行为,采用SEC-HPLC检测制备前后蛋白的聚集程度,并与不含多糖的BSA纤维做对照。结果:添加Dextran以后蛋白的包封率由52.68%提高到63.92%,第一天突释不大于药物载量的15%,对蛋白单体的保持达到85%以上。结论:Dextran可以改善一般组织工程纤维中蛋白药物的释放,提高蛋白药物在制剂、贮存、释放过程中的稳定性,增加纤维的载药量。
Objective: To study the effect of Dextran on the release of protein drugs. Methods: The model protein BSA was dissolved in polysaccharide solution, and the sustained release fibers were prepared by electrospinning by W / O emulsion method. MicroBCA method was used to determine the in vitro release behavior of the fibers. The degree of aggregation before and after preparation was detected by SEC-HPLC and compared with BSA fibers without polysaccharides. Results: The encapsulation efficiency of Dextran increased from 52.68% to 63.92% after the addition of Dextran. The first day of burst release was no more than 15% of the drug load and the retention of protein monomer was over 85%. Conclusion: Dextran can improve the release of protein drugs in general tissue engineering fibers and improve the stability of protein drugs during preparation, storage and release, and increase the drug loading of fibers.