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我国很多饮用水源水已检测出雌激素受体干扰效应,但是其他效应如雄激素受体干扰效应却罕见报道。为了弥补这方面资料的缺乏,应用雄激素受体双杂交酵母结合S9代谢方法评价了我国6大流域23个水源地水源水以及3个供水厂不同工艺出水样品的雄激素受体干扰效应。结果显示,所有水源水样品浓缩液均没有检测出雄激素受体直接或间接诱导效应,但都观察到了抑制效应,抑制效应以抑制剂氟他胺当量浓度进行表征:直接抑制效应在0.67~3.68μg·L-1之间,间接抑制效应在0.52~3.02μg·L-1之间;长江三角洲和淮河流域水源地的水源水雄激素抑制效应明显高于其他流域;经S9代谢后,有16个水源地的氟他胺当量比代谢前降低。3个饮用水厂处理工艺能够使源水的雄激素受体抑制效应降低19.1%~70.5%。研究表明,我国水源水中普遍含有雄激素受体干扰效应物质,目前水厂常规处理工艺对雄激素受体干扰效应有一定的去除效果,但不能完全去除。雄激素受体双杂交酵母测试能够快速、综合地对水体中的雄激素受体干扰效应进行评价,是饮用水安全评价的重要补充手段。
Many sources of drinking water in our country have detected estrogen receptor interference effects, but other effects such as androgen receptor interference effects are rarely reported. In order to make up for the lack of information in this aspect, the androgen receptor two-hybrid yeast combined with S9 metabolic method was used to evaluate the effects of androgen receptor on the water samples from 23 drinking water sources and three waterworks in China’s six major drainage areas. The results showed that all water source water samples did not detect direct or indirect induction of androgen receptor concentration, but both inhibitory effects were observed and the inhibitory effect was characterized by the inhibitor equivalent concentration of flutamide: the direct inhibitory effect was between 0.67 and 3.68 μg · L-1, and the indirect inhibitory effect was between 0.52 ~ 3.02μg · L-1. The androgen inhibitory effect of water and source water in the Yangtze River Delta and Huaihe River Basin was significantly higher than that in other basins. After S9 metabolism, 16 Flutamide equivalent to a water source lower than before metabolism. Three drinking water treatment plants can reduce the androgen receptor inhibitory effect of source water by 19.1% -70.5%. Studies have shown that water sources in our country generally contain androgen receptor interfering substances. At present, the conventional treatment process of waterworks has a certain effect of removing the androgen receptor interference effect, but it can not be completely removed. Androgen receptor two-hybrid yeast test can rapidly and comprehensively evaluate the effect of androgen receptor interference in water, which is an important complement to drinking water safety evaluation.