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目的探讨荜茇酰胺(piperlongumine)对胶质瘤增殖影响及机制。方法采用荜茇酰胺处理2种不同人脑胶质瘤细胞系,同时设正常神经元和胶质细胞对照;采用噻唑蓝法检测细胞增殖力,采用试剂盒法检测细胞内活性氧(ROS)及过氧化物还原酶4(PRDX4)活性,采用Annexin V染色法检测细胞凋亡率,采用RT-PCR法检测PRDX1~6基因表达。结果与对照组比较,荜茇酰胺能够明显抑制胶质瘤细胞增殖能力,增强2种胶质瘤细胞内的ROS水平(P<0.05),但对正常神经元则无明显影响;与对照组比较,胶质瘤细胞凋亡率明显升高(P<0.05),胶质瘤细胞内PRDX4表达上升(P<0.05)。结论荜茇酰胺可通过提高PRDX4的表达从而介导胶质瘤细胞的凋亡。
Objective To investigate the effect of piperlongumine on the proliferation of glioma and its mechanism. Methods Two kinds of human glioma cell lines were treated with 荜 茇 amide, and normal neurons and glial cells were also established. Cell proliferation was measured by thiazolyl blue assay. Reactive oxygen species (ROS) and intracellular reactive oxygen species Peroxiredoxin 4 (PRDX4) activity was detected by flow cytometry. Annexin V staining was used to detect the apoptosis rate. The expression of PRDX1 ~ 6 gene was detected by RT-PCR. Results Compared with the control group, 荜 茇 amide significantly inhibited the proliferation of glioma cells and increased the levels of ROS in the two glioma cells (P <0.05), but had no significant effect on normal neurons. Compared with the control group , The apoptosis rate of glioma cells was significantly increased (P <0.05), and the expression of PRDX4 in glioma cells increased (P <0.05). Conclusion Gallactam can mediate the apoptosis of glioma cells by increasing the expression of PRDX4.