论文部分内容阅读
以蒸馏水中的糖原悬浮液为参照物，测定了胭脂红酸的荧光发射寿命，τ＝０．０９３±０．０１０ｎｓ．研究了ＯＨ－和Ｉ－对胭脂红酸荧光的猝灭，后者遵守Ｓｔｅｒｎ－Ｖｏｌｍｅｒ方程，其猝灭常数ＫＱ＝１．２７±０．０９Ｌ·ｍｏｌ￣（－１），前者则不是遵守此方程的简单荧光猝灭，这可能是由于胭脂红酸中酚基的存在降低了最低激发态能量的原因。
Fluorescence emission lifetime of carminic acid was measured with a glycogen suspension in distilled water as a reference, τ = 0.093 ± 0.010 ns. The quenching of the fluorescence of carminic acid by OH- and I- was studied. The latter was in accordance with the Stern-Volmer equation with a quenching constant KQ = 1.27 ± 0.09 L · mol -1, the former did not obey this The simple fluorescence quenching of the equation may be due to the reduction of the lowest excited state energy due to the presence of a phenolic group in carminic acid.