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当１６名正常受试者从最低音、中音、高音及假声发元音“ｉ”时，对其环甲肌、甲杓肌做了肌电图检查。同时对２０名音乐学院声乐系学生整个音域的频率、声强、声门下压力、气流率进行了测量。结果表明：随着发音频率的升高，环甲肌及甲杓肌的电活动逐渐增强。在最低音发音时环甲肌的电活动较小，没有甲杓肌强。在高音及假声发音时，环甲肌电活动最强。尤其在假声音域，环甲肌与甲杓肌共同协同作用来完成对频率的调节。当发音频率逐渐升高时，声强及声门下压力同样也逐渐升高，呈正相关（Ｐ＜０．０１）。频率与气流率也为正相关（Ｐ＜０．０１，除２个男低音之外）。有１０名受试者在发高音和假声时，气流率的值呈现零值和负值。声门下压力及喉内肌的电活动在调节基频中起到了重要的作用，气流率在调节基频中也有一定的作用，但在发高音和假声时，有１／２的受试者的气流率几乎没有起作用，呈现零值或负值。
When 16 normal subjects from the bass, tenor, treble and fallacious vowel “i”, its ring armor, thyroid muscle EMG examination. At the same time, the frequency, sound intensity, subglottic pressure and airflow rate of the entire vocal music system of 20 music colleges were measured. The results showed that the electrical activity of the cricothyroid muscle and the thyrotropin gradually increased with the increase of the pronunciation frequency. In the lowest sound ring when the electrical activity of the smaller, there is no strong muscle. In the treble and falsetto pronunciation, the most intense ring electro-muscular electromyography. Especially in the fake sound domain, the ring armor and armor muscle synergies to complete the coordination of the frequency. The sound intensity and subglottic pressure also increased gradually as the pronunciation frequency increased gradually (P <0.01). Frequency and airflow rate were also positively correlated (P <0.01, except for 2 bass). When 10 subjects were on treble and falsetto, the airflow rate showed zero and negative values. Subglottic pressure and electrical activity of the throat muscles play an important role in regulating the fundamental frequency, and the air flow rate also plays a role in adjusting the fundamental frequency, but in the treble and fake sounds, 1/2 of the subjects Gas flow rate almost no effect, showing zero or negative value.