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红掌细菌性疫病(Xanthomonas axonopodis pv.dieffenbachiae,简称Xad)是红掌等天南星科花卉毁灭性病害,该病通过带菌种苗调运不断在我国扩散蔓延,国内尚未有检测方法。通过筛选和重新设计引物,建立了Xad的PCR检测方法,结果表明,利用引物Xad-F/Xad-R进行PCR,能扩增出检测Xad的特异性DNA片断,其灵敏度可达1×102CFU/mL,DNA的最低检出限为0.44 ng/μL,可用于红掌苗的带菌检测和红掌细菌病害的鉴定。
Anthurium (Xanthomonas axonopodis pv.dieffenbachiae, referred to as Xad) is the anthurium and other Aconitum flowers devastating disease, the disease is transported through the carrier with bacteria continue to spread in our country, there is no test method in China. The Xad PCR method was established by screening and redesigning the primers. The results showed that Xad-specific Xad-specific DNA fragments could be amplified by PCR using primers Xad-F / Xad-R with the sensitivity of 1 × 102CFU / mL, the detection limit of DNA is 0.44 ng / μL, which can be used for the detection of the infection of Anthurium andraeanum and the identification of the diseases of Anthurium andrrhus.