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一、材料与方法1、菌种的制备:以中国科学院微生物研究所分离的“5.69”纯菌种—紫芝,作二级(一级斜面菌种,二级木屑培养基菌种)扩大培养。培养基的配方、灭菌、接种、培养过程均同瓶栽灵芝。所不同的是第二级扩大培养中,只完成菌丝体快速生长发育阶段,即作为生产用的菌种。2、培养材料的选择和处理:将培养黑木耳、香菇所废弃的麻栗枝梢,头年置于露地,任其风吹、日晒、雨淋,直至略成半朽状态,次春截成约10厘米的枝段,过粗的劈成小片,用清水浸泡1—2昼夜备用。
I. Materials and methods 1. Preparation of the strain: The “5.69” pure strain - Ganoderma lucidum isolated from Institute of Microbiology, Chinese Academy of Sciences, was expanded for secondary culture (primary bevel strain, secondary sawdust culture). Medium formula, sterilization, inoculation, culture process are the same bottle of Ganoderma lucidum. The difference is the second level to expand the cultivation, only to complete the mycelium rapid growth and development stage, that is, as the production of bacteria. 2, training material selection and processing: Will cultivate black fungus, mushrooms abandoned Thistle branches, the first year placed in the open, let the wind, sun, rain, until slightly into a semi-decadent state, the second spring cut Into about 10 cm of branches, too thick split into small pieces, soaked with water 1-2 days and nights spare.