恩格尔哈特Знгедьгард氏及斯米尔诺娃Смир-(?)ова氏法的优点是血清用量较少(用0.1毫升,假如做平行测定时用0.2毫升),因此在必要时可从手指采血,还可以用0.1毫升全血来测定(但全血与血清之胆固醇含量颇不一致)。原理:胆固醇溶液在气仿溶液中与醋酸酐及硫酸作用而呈绿色,然後与同样处理之已知标准液比色之。 The advantages of Engelhardt Знгедьгард’s and Smirnova Смир-(?) ова’s method are that the amount of serum used is small (0.1 ml, if parallel assay is used 0.2 ml), so blood can be taken from the finger if necessary. It can be measured with 0.1 ml whole blood (but the blood cholesterol is quite different from the serum). Principle: Cholesterol solution reacts with acetic anhydride and sulfuric acid in the chloroform solution and becomes green. It is then compared with the known standard solutions treated in the same way.