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目前作梅毒血清反应,主要用牛心肌酒精浸出液制抗原,但为了增强抗原敏感性,可加胆固醇(0.25——0.3％)。近年工作証明,与胆固醇結合之磷脂类(磷脂与卵磷脂),是梅毒血清反应活动的基础,而三者必需适当配合,才能得到敏感性及特异性均高的抗原。磷脂类广泛分布于动植物界,被采用的有黄豆,小麦,馬鈴薯,核桃及巴旦杏仁等。我們制造植物性抗原,开始用五倍于植物的乙醇浸提两周后。在滤液中加胆固醇(0.25％),此法所得抗原,其抗科体及非特异性之百分此均甚高。
At present, for the serum syphilis reaction, the main use of bovine cardiac alcohol leaching liquid preparation of antigens, but in order to enhance antigen sensitivity, you can add cholesterol (0.25 - 0.3%). Recent work has shown that the phospholipids (phospholipids and lecithins) bound to cholesterol are the basis of syphilis serum activity, and the three must be properly coordinated to obtain antigens with high sensitivity and specificity. Phospholipids are widely distributed in animal and plant kingdoms. Soybeans, wheat, potatoes, walnuts and almonds are used. We make plant antigens and start leaching with ethanol five times the plant two weeks later. Cholesterol (0.25%) was added to the filtrate. Antigens and non-specificity of antigens obtained by this method were very high.