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木薯是热带典型的高淀粉、抗逆、抗旱作物。可溶性淀粉合成酶(SSS)是植物淀粉合成过程中的关键酶之一。根据已发表的拟南芥SSSⅣ基因序列,通过同源比对从木薯AM560、KU50基因组中获得木薯MeSSSⅣ基因的cDNA序列以及该基因部分启动子序列,设计特异引物,从木薯栽培种KU50基因组DNA中克隆了MeSSSⅣ基因系列1 068 bp(该序列包含978 bp的启动子调控序列及90 bp的基因编码序列)。序列分析表明,启动子调控序中除含有典型的真核生物核心启动子区域外,还含有多个TATA-box、CAAT-box等启动子元件以及多种与脱水、激素和光响应相关的顺式作用元件,特别是发现CGACGOSAMY3、DOFCOREZM、SREATMSD、SURE等与糖信号相关的重要顺式元件,这些元件预示MeSSSⅣ基因的表达可能与糖信号相关。以上结果说明,MeSSSⅣ可能参与木薯多种代谢过程,其表达可能受多种调控因子的调节。
Cassava is tropical typical high starch, anti-stress, drought-resistant crops. Soluble starch synthase (SSS) is one of the key enzymes in plant starch synthesis. According to the published sequence of Arabidopsis thaliana SSSⅣ gene, the cDNA sequence of cassava MeSSSⅣ gene and partial promoter sequence of cassava were obtained by homology alignment from the cassava AM560 and KU50 genomes. Specific primers were designed from the genomic DNA of Cauliflower cultivar KU50 The 1 068 bp gene sequence of MeSSSⅣ was cloned, which contains a 978 bp promoter sequence and a 90 bp coding sequence. Sequence analysis showed that the promoter regulatory sequence contained many promoter elements such as TATA-box and CAAT-box in addition to the typical eukaryotic core promoter region, as well as various cis-acting regulators related to dehydration, hormone and light response It is found that the important cis elements related to sugar signal such as CGACGOSAMY3, DOFCOREZM, SREATMSD, SURE and so on indicate that the expression of MeSSSⅣ gene may be related to the sugar signal. The above results indicate that MeSSSⅣ may be involved in various metabolic processes of cassava and its expression may be regulated by a variety of regulatory factors.