背景与目的:PTEN基因是新发现的一种抑癌基因,定位于人染色体10q23。它的缺失和突变可能构成一个新的信号转导途径,与人类恶性肿瘤的发生普遍相关。资料报道,肾细胞癌有抑癌基因PTEN的缺失和突变。但目前尚未见到关于抑癌基因PTEN在肾细胞癌中表达的研究,本实验研究抑癌基因PTEN在肾细胞癌的表达和生物学意义。方法:应用免疫组织化学SP法检测5例正常肾组织、18例癌旁肾组织和40例肾细胞癌组织中抑癌基因PTEN的表达;分析抑癌基因PTEN的表达与肾细胞癌病理类型、淋巴结转移之间的关系。结果:5例正常肾组织和18例癌旁肾组织均有较强的PTEN蛋白的表达,正常肾组织和癌旁肾组织PTEN蛋白的表达强度、阳性细胞的分布形式无明显差异。肾小管上皮细胞的胞质呈PTEN蛋白阳性,免疫组化染色程度较强,肾小球无PTEN蛋白的表达。抑癌基因PTEN在肾细胞癌中的表达不同于正常肾组织和癌旁肾组织,12.5%呈PTEN蛋白阴性,17.5%呈弱阳性,70%呈阳性或强阳性。PTEN蛋白阴性、弱阳性和阳性的肾细胞癌,肾门淋巴结转移率分别为80%,51.74%,10.71%。PTEN蛋白阴性和弱阳性肾细胞癌的肾门淋巴结转移率与PTEN阳性者比较,差异有显著性(P<0.05)。结论:本实验提示肾细胞癌中存在着抑癌基因PTEN的表达缺失和异常,可能与肾细胞癌的发生、发展有关 BACKGROUND & AIM: The PTEN gene is a newly discovered tumor suppressor gene located on human chromosome 10q23. Its absence and mutation may constitute a new signal transduction pathway, which is generally associated with the occurrence of human malignant tumors. Data reported, renal cell carcinoma suppressor gene PTEN deletion and mutation. However, no study has been done yet on the expression of tumor suppressor gene PTEN in renal cell carcinoma. This study investigated the expression and biological significance of tumor suppressor gene PTEN in renal cell carcinoma. Methods: Immunohistochemical SP method was used to detect the expression of tumor suppressor gene PTEN in 5 normal renal tissues, 18 adjacent tissues and 40 renal cell carcinoma tissues. The expression of tumor suppressor gene PTEN and the pathological type of renal cell carcinoma were analyzed. The relationship between lymph node metastasis. Results: The expression of PTEN protein in 5 cases of normal renal tissues and 18 cases of adjacent tissues of para-cancerous tissues was strong. There was no significant difference in the expression intensity of PTEN protein and the distribution of positive cells in normal renal tissues and paracancer tissues. The cytoplasm of renal tubular epithelial cells was positive for PTEN protein, strong immunohistochemical staining, glomerular PTEN protein expression. The expression of tumor suppressor gene PTEN in renal cell carcinoma was different from that in normal renal tissues and paracancer renal tissues. PTEN protein was negative in 12.5%, weakly positive in 17.5% and positive in 70%. PTEN protein negative, weak positive and positive renal cell carcinoma, renal cell lymph node metastasis rates were 80%, 51.74%, 10.71%. There was significant difference (P <0.05) between the rate of renal lymph node metastasis and PTEN positive in PTEN negative and weak positive renal cell carcinoma. Conclusion: This study suggests that there is a loss of expression of tumor suppressor gene PTEN and abnormalities in renal cell carcinoma, which may be related to the occurrence and development of renal cell carcinoma