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以赤霉素(GA)处理后的G2豌豆为材料,构建了一个2.0×106的cDNA文库,用随机筛选法从该cDNA文库中得到一个完整的cDNA.其中1115bp全序列分析表明,它编码了豌豆5S核糖体RNA(5SribosomalRNA,5SrRNA),基因内部存在着与核糖体蛋白L5及5SrRNA结合蛋白(5SrRNABindingProtein)同源的区段,这些同源区段是蛋白与RNA结合的位点.基因内部还存在着大量的重复序列.
A 2.0 × 106 cDNA library was constructed from GA (GA) -treated G2 pea, and a complete cDNA was obtained from the cDNA library by random screening. Among them, 1115bp full-sequence analysis indicated that it encodes 5S ribosomal RNA (5SrRNA) of pea and has internal homology with ribosomal protein L5 and 5SrRNA binding protein (5SrRNABindingProtein). These homologous segments are proteins Sites that bind to RNA. Genes also exist within a large number of repetitive sequences.