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目的观察亚氨基二丙腈(iminodipropionitrile,IDPN)和2,5-二甲氧-4-碘苯-2-氨基丙烷(±-1-2,5-dimethoxy-4-iodophenyl-2-aminopropane,DOI)诱导的抽动障碍(tic disorder,TD)动物模型的运动抽动和刻板行为表现,为选择可以更好地模拟TD特征性行为的造模方法提供依据。方法 SD大鼠30只随机分3组,每组10只。IDPN组大鼠给予IDPN 150mg/kg腹腔注射,连续7d,DOI组大鼠给予DOI 1mg/kg腹腔注射,连续15d,正常组给予生理盐水腹腔注射,连续7d,给药体积均为1mL/100g。造模结束后记录各组大鼠在造模结束第1至第7天、第14天和第28天的运动行为评分、刻板行为评分和分类刻板行为评分。结果 IDPN组和DOI组大鼠在第1至第4天的运动评分、刻板行为评分,除理毛、舔咬笼子以外的分类刻板行为评分均高于正常组,且IDPN组大鼠在第5、6、7、14、21、28天的运动评分和第5、7、14、21、28天的刻板行为评分均高于正常组和DOI组,差异均有统计学意义(P<0.05)。IDPN组大鼠的吃木屑、旋转、摆头、舞蹈样运动等分类刻板运动评分均高于DOI组,DOI组大鼠口爪运动、自咬等分类刻板运动评分均高于IDPN组,差异均有统计学意义(P<0.05)。结论 IDPN模型与DOI模型均可以模拟抽动患者的兴奋性增高,活动增多和抽动等症状,表观效度较好。与DOI模型相比较,IDPN模型具有建模时间短,模型维持时间长久,且花费较少等优势。
Objective To observe the effects of iminodipropionitrile (IDPN) and 2,5-dimethoxy-4-iodophenyl-2-aminopropane ) Induced tic disorder (TD) animal models of motor tics and stereotyped behavior, to provide a basis for selecting modeling methods that can better simulate TD characteristic behavior. Methods Thirty SD rats were randomly divided into three groups of 10 rats. Rats in IDPN group were intraperitoneally injected with 150 mg / kg IDPN for 7 days. Rats in DOI group were intraperitoneally injected with DOI 1 mg / kg for 15 days. Normal rats were injected intraperitoneally with physiological saline for 7 days. The volume of drug administered was 1 mL / 100 g. At the end of modeling, the scores of locomotor activity score, stereotyped behavior score and categorized stereotyped behavior score of rats in each group were recorded on the 1st to 7th days, the 14th and 28th days after the end of the modeling. Results The scores of motor score, stereotyped behavior, classification of stereotyped behavior except for grooming and licking cages in IDPN group and DOI group were higher than those in normal group on day 1 to 4, , 6,7,14,21,28 days of exercise score and the 5th, 7th, 14th, 21st and 28th day of stereotypical behavior score were higher than the normal group and DOI group, the difference was statistically significant (P <0.05) . In IDPN group, the scores of stereotyped motor activity such as sawdust, rotation, swinging, dance-like exercise in DOI group were higher than those in DOI group. The scores of paw movement and self-bite in DOI group were higher than those in IDPN group There was statistical significance (P <0.05). Conclusion Both IDPN model and DOI model can simulate tachycardia patients with increased excitability, increased activity and tics and other symptoms, the apparent validity is better. Compared with the DOI model, IDPN model has the advantages of short modeling time, long model maintenance time and less cost.