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目的研究不同时期HIV感染者血液中的病毒载量与T细胞亚群之间关系。方法选取62例HIV感染者和10例健康人为对照,感染者依据病程时期分为3组,采用荧光PCR检测技术测定血液中HIV病毒载量和流式细胞仪计数外周血中CD3~+、CD4~+、CD8~+淋巴细胞。通过分析得出之间关系。结果处于不同疾病阶段的(无症状感染者、有症状感染者和AIDS病人)的血浆病毒载量不同,组间有统计学意义(P<0.05),艾滋病组的病毒载量最高为4.32×106,远高于其他组,3组中的CD4~+细胞、CD4~+/CD8~+比值下降且明显低于正常人群(P<0.05)具有统计学意义。各因素的相关性分析显示:HIV病毒载量与CD3~+、CD4~+细胞及CD4~+/CD8~+比值之间有高度直线负相关关系,与CD8+细胞无直接相关性。结论不同时期的HIV感染者其病毒载量水平、免疫学状况有明显不同。当HIV病毒载量升高时,CD4~+细胞、CD4~+/CD8~+比值下降,提示疾病处于进展中。因此,检测这些指标变化可为HIV的临床分期、判断预后和治疗提供依据。
Objective To study the relationship between viral load and T cell subsets in the blood of HIV infected persons in different periods. Methods Sixty-two HIV-infected patients and 10 healthy controls were selected as controls. The infected patients were divided into three groups according to their duration of disease. Fluorescent PCR was used to detect the HIV viral load and flow cytometry to count the levels of CD3 +, CD4 ~ +, CD8 ~ + lymphocytes. By analyzing the relationship between. Results The plasma viral load at different disease stages (asymptomatic, symptomatic and AIDS) was different, with statistical significance (P <0.05). The highest viral load in AIDS group was 4.32 × 106 , Which was much higher than that of other groups. The ratio of CD4 ~ + / CD8 ~ + in CD4 ~ + cells in three groups was significantly lower than that in normal controls (P <0.05). Correlation analysis showed that there was a highly linear negative correlation between HIV viral load and CD3 ~ +, CD4 ~ + cells and CD4 ~ + / CD8 ~ + ratio, but not with CD8 + cells. Conclusions The viral load level and the immunological status of HIV infected persons in different periods have obvious differences. When the HIV viral load increased, CD4 ~ + cells, CD4 ~ + / CD8 ~ + ratio decreased, suggesting that the disease is in progress. Therefore, detecting changes in these indicators may provide the basis for the clinical stage of HIV, prognosis and treatment.