通过将野生型p53(wt-p53)基因与荧光蛋白(GFP)基因的融合,导入高转移肝癌细胞中,并利用荧光蛋白的示踪作用,观察p53基因在肝癌细胞中的正常表达及定位情况.根据野生型p53基因的编码顺序,PCR方法扩增突变的wt-p53基因,使其3’端的终止密码TGA突变为TGG;用基因重组技术将其与GFP融合,通过真核表达质粒-脂质体介导,导入人高转移肝癌细胞系MHCC97(PCR检测有p53基因突变)中.用荧光显微镜观察p53的表达情况.实验表明, The fusion of wild-type p53 (wt-p53) gene and fluorescent protein (GFP) gene was introduced into high metastatic hepatoma cells, and the normal expression and localization of p53 gene in hepatoma cells were observed by using the fluorescent protein tracer function. Based on the coding sequence of the wild-type p53 gene, the mutant method was used to amplify the mutant wt-p53 gene, and the 3’-end stop-gap TGA was mutated to TGG; it was fused with GFP by gene recombination technology and expressed in eukaryotic expression plasmid-lipid. Plastid-mediated introduction of human high-metastatic liver cancer cell line MHCC97 (p53 gene mutation detected by PCR). The expression of p53 was observed by fluorescence microscopy. Experiments showed that