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在明确瓜类白粉病生理小种的基础上,以感白粉病新疆哈密瓜[Cucumis melo L.ssp.melo convar.ameri(Pang.)Greb.]来源的自交系K7-2和抗白粉病日本网纹甜瓜[Cucumismelo L.ssp.melo convar.cantalupa(Pang.)Greb.]来源的自交系K7-1为亲本及其F2S6群体为试材,对甜瓜白粉病抗性遗传机制和紧密连锁标记进行深入研究。群体遗传分析表明,K7-1对白粉病Podosphaera xanthii(DC.)VPGelyuta生理小种2F的抗性受一对显性基因Pm-2F控制。同时采用SSR分析技术发现,抗病特异片段CM-BR120-172、CMBR8-98与Pm-2F紧密连锁,连锁距离分别为1cM和3cM。抗病特异片段CMBR120-172在120份甜瓜种质资源材料验证中符合率达87.5%。
Based on the identification of physiological races of melon powdery mildew, the inbred lines K7-2 derived from powdery mildew of Xinjiang Hami melon [Cucumis melo L.ssp.melo convar. Meri (Pang.) Greb.] And the powdery mildew resistant Japanese The inbred line K7-1 derived from Cucumis melo L.ssp.melo convar.cantalupa (Pang.) Greb. Was used as parents and its F2S6 population as test material, and the genetic mechanism of resistance to melon powdery mildew and the close linkage markers Conduct in-depth study. Population genetic analysis showed that the resistance of K7-1 to VPGelyuta 2F of powdery mildew of Podosphaera xanthii (DC.) Was controlled by a dominant gene Pm-2F. At the same time using SSR analysis found that disease-specific fragments of CM-BR120-172, CMBR8-98 and Pm-2F closely linked, the linkage distance were 1cM and 3cM. The resistance-specific fragment CMBR120-172 conforms to 87.5% of the 120 melon germplasm resources.