论文部分内容阅读
于1985年通过山杨(Populus davidiano)形成层、顶芽、腋芽的组织培养,成功的获得了再生植株,为当前生产大量繁殖山杨无性系优质苗提供了新的手段。 通过愈伤组织途径诱导再生植株,是以形成层为材料。诱导愈伤组织的培养基为ACM+BA_1+NAA_2或2.4—D_2mg/l+蔗糖2％,愈伤组织诱导率为73.6％,结构致密;诱导器官分化培养基为ACM或1/2MS+BA_(0.5)mg/l+NAA_(0.05-0.01)mg/l+蔗糖2％,最佳分化率为83.3％。 通过芽培养诱导再生植株,是以顶芽腋芽为材料,利用7个组合培养基试验比较,诱导芽分化最佳培养基为ACM+BA_(0.3-0.5)mg/l+NAA_(0.02-0.05)mg/l+蔗糖2％;适宜取材期为萌动期,其次是休眠期。 诱导初期休眠芽的分化,在适宜激素培养基中,另加20mg/l的精氨酸,芽的分化率有较明显的提高。 诱导生根培养基为1/2MS或ACM+IBA_(0.3-0.5)mg/r+糖蔗2％,生根率达77.8％。
In 1985, the regenerated plants were successfully obtained through the tissue culture of Populus davidiano cambium, apical bud and axillary bud, which provided a new method for the current production of high-quality clonal poplar aspen. Regeneration plants are induced by callus pathways, using the cambium as a material. The medium of induced callus was ACM + BA_1 + NAA_2 or 2.4-D_2mg / l + sucrose 2%, the callus induction rate was 73.6%, and its structure was dense. The induced organs differentiation medium was ACM or 1 / 2MS + BA 0.5 ) mg / l + NAA_ (0.05-0.01) mg / l + 2% sucrose, the best differentiation rate was 83.3%. The optimal medium for inducing bud differentiation was ACM + BA_ (0.3-0.5) mg / l + NAA_ (0.02-0.05), which was induced by bud culture. mg / l + sucrose 2%; suitable for the germination period, followed by the dormant period. Induction of early dormant bud differentiation, in the appropriate hormone medium, plus 20mg / l of arginine, bud differentiation rate was significantly improved. The rooting medium was 1 / 2MS or ACM + IBA (0.3-0.5) mg / r + 2% sugarcane, and the rooting rate was 77.8%.