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目的:探究硫化氢(Hydrogen sulfide，Hn 2S)对人胃黏膜细胞GES-1(gastric mucosal epithelial，GES-1)增殖的影响。n 方法:将不同浓度(0，25，100，400，800和1600 μmol/L)的硫氢化钠(NaHS)分别作用于胃黏膜GES-1细胞24、48和72 h并按此分组，噻唑蓝[3-(4，5-Dimethylthiazol-2-yl)-2，5-diphenyltetrazolium bromide, MTT]法检测各组细胞的增殖水平，酶联免疫吸附实验(enzyme-linked immunosorbent assay, ELISA)检测细胞内Hn 2S的含量。n 结果:MTT结果显示，浓度为800 μmol/L的NaHS作用胃黏膜细胞GES-1 24 、48、72 h时，增殖率分别为(97.67±5.03)%、(77.66±3.51)%、(63.00±2.00)%。浓度为1600 μmol/L的NaHS作用胃黏膜细胞GES-1 24、48、72 h时，增殖率分别为(89.67±7.51)%、(70.00±4.00)%、(54.00±2.00)%。ELISA结果显示，当浓度为800 μmol/L的NaHS作用胃黏膜细胞GES-1 24、48和72 h时，GES-1产生的Hn 2S浓度分别分别为(8.73±0.44)、(13.22±0.86)、(17.31±1.05)μmol/L。当浓度为1600 μmol/L的NaHS作用胃黏膜细胞GES-1 24 、48、72 h时，GES-1产生的Hn 2S浓度分别为(13.15±0.46)、(19.01±0.28)、(22.68±0.65)μmol/L。统计分析显示，当浓度为800 μmol/L和1600 μmol/L的NaHS作用胃黏膜细胞GES-1时，48 h作用组和72 h作用组与24 h作用组比较，胃黏膜细胞GES-1增殖均降低，差异均具有统计学意义(n P值均<0.05)；胃黏膜细胞GES-1产生的Hn 2S浓度明显增高，差异均具有统计学意义(n P值均<0.05)。此外，细胞的增殖与NaHS浓度呈负相关(n r ＝ -0.558，n P<0.05)，细胞产生的Hn 2S浓度与NaHS浓度呈正相关(n r＝0.868，n P<0.05)，且细胞的增殖与细胞产生的Hn 2S浓度具有负相关(n r ＝-0.863，n P<0.05)。n 结论:浓度为800 μmol/L和1600 μmol/L的NaHS能诱导GES-1细胞产生Hn 2S，抑制GES-1细胞增殖，且呈浓度依赖性。n “,”Objective:To explore the effect of different concentrations of Hydrogen sulfide (Hn 2S) on the proliferation in gastric mucosal epithelial (GES-1) cells.n Methods:After GES-1 cells were treated with different concentrations of NaHS (0, 25, 100, 400, 800 and 1600 μmol/L)for 24, 48 and 72 h, the proliferation of GES-1 cells was detected by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, and the concentrations of H n 2S in GES-1 cells were determined by enzyme-linked immunosorbent (ELISA) assay.n Results:MTT assay showed that the cell proliferation rate was 97.67%±5.03%, 77.66%±3.51% and (63.00±2.00)% after GES-1 cells were treated with 800 μmol/L NaHS for 24, 48 and 72 h and the cell proliferation rate was (89.67±7.51)%, (70.00±4.00)% and (54.00±2.00)%. After GES-1 cells were treated with 1600 μmol/L NaHS for 24, 48 and 72 h. Moreover, ELISA assay showed that the concentrations of H n 2S in cells were(8.73±0.44)、(13.22±0.86)and(17.31±1.05)μmol/L after GES-1 cells were treated with 800 μmol/L NaHS for 24, 48 and 72 h and the concentrations of Hn 2S in cells was (13.15±0.46), (19.01±0.28) and (22.68±0.65)μmol/L after GES-1 cells were treated with 1600 μmol/L NaHS for 24, 48 and 72 h. Statistical analysis showed that compared with 24 h, the proliferation of GES-1 cells were significantly lower (alln P values <0.05) and the concentrations of H n 2S were increased than those of 48 and 72 h when the GES-1 cells were treated with 800 μmol/L and 1600 μmol/L NaHS respectively(all n P values <0.05) . In additional, the GES-1 cells proliferation was negatively correlated with the NaHS concentration ( n r =-0.558, n P<0.05). On the contrary, the Hn 2S concentration produced by the GES-1 cells was positively correlated with the NaHS concentration (n r=0.868, n P<0.05). Moreover, the GES-1cell proliferation was negatively correlated with the Hn 2S concentration produced by GES-1 cells (n r=-0.863, n P<0.05).n Conclusion:NaHS with concentration of 800 μmol/L and 1600 μmol/L can induce GES-1 cells to produce H n 2S and inhibit GES-1 cells proliferation in a dose manner.n