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目的建立茯苓中茯苓酸的测定方法,比较不同菌种培育的茯苓中茯苓酸的量,为评价不同菌株培育茯苓的品质提供理论依据,从而确定优良菌种,控制茯苓资源品质。方法采用反相高效液相色谱法(RP-HPLC),Kromasil 100-5C18色谱柱(250mm×4.6mm,5μm),柱温30℃,流动相为乙腈-0.2%甲酸(80∶20),体积流量1.0mL/min,检测波长242nm。结果茯苓酸在0.48～2.40μg与其色谱峰面积呈良好线性关系(r=0.9997),平均回收率100.26%,RSD为1.26%。不同菌种培育茯苓中茯苓酸的量有所差异。结论该方法简便、专属、稳定,可用于评价不同菌种培育茯苓中茯苓酸量的研究。
Objective To establish a method for the determination of Poria cocos in Poria cocos, to compare the amount of Poria cocos acid in Poria cocos with different strains and to provide a theoretical basis for evaluating the quality of Poria cocos with different strains so as to determine the fine strains and control the quality of Poria cocos. Methods RP-HPLC was performed on a Kromasil 100-5C18 column (250 mm × 4.6 mm, 5 μm) with a column temperature of 30 ° C and a mobile phase of acetonitrile-0.2% formic acid (80:20) Flow 1.0mL / min, detection wavelength 242nm. Results Porosity acid showed a good linearity (r = 0.9997) between 0.48 ~ 2.40μg and its peak area, with an average recovery of 100.26% and a RSD of 1.26%. Different strains of Poria cultivating in the amount of Poria acid are different. Conclusion The method is simple, specific and stable and can be used to evaluate the contents of Poria cocos in different strains of Fuling.