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用24～26天龄的大白鼠腹腔感染10~4～10~5个己感染伯氏鼠疟原虫的红细胞,每7天用幼鼠常规转种1次。培养用的疟原虫取自感染后4～5天的鼠血,即以无菌操作抽取心血置于加有肝素的磷酸盐缓冲液中(0.1M磷酸钠缓冲液,每毫升含肝素16个国际单位,pH 7.4)。另从1只未感染的幼鼠同样取血。分别将两鼠的血在30℃内以450g离心6分钟,并以9倍容量的培养液洗涤(培养液含199培养基1克,葡萄糖200毫克,次黄嘌呤核苷2毫克,三磷酸腺苷1毫克,青霉素500单位,庆大霉素0.4毫克,肝素200单位,除
Ten to four to ten to five mouse erythrocytes infected with P. murine parasite were infected intraperitoneally with 24 to 26 day-old rats, and the rats were routinely transplanted once every 7 days. Plasmodium falciparum was taken from rat blood 4 to 5 days after infection, ie blood was drawn aseptically into heparinized phosphate buffer (0.1 M sodium phosphate buffer, 16 heparin per ml Unit, pH 7.4). The other one from uninfected young mice were also bled. The blood from both rats was centrifuged at 450 g for 6 minutes at 30 ° C and washed with a 9-fold volume of culture medium containing 1 g of 199 medium, 200 mg of glucose, 2 mg of inosine and 1 mg of adenosine triphosphate 500 units of penicillin, 0.4 mg of gentamicin, 200 units of heparin, except