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用等电点聚焦分离技术研究小鼠组织提取物的丙酮酸盐激酶的同功酶谱,发现与大鼠组织的酶谱极为相似。而正常小鼠肝和艾氏腹水癌宿主肝的提取物作等电点聚焦分离技术分析或动力学测定,表明后者有较高比例的丙酮酸盐激酶 K 型同功酶。用艾氏腹水癌细胞匀浆经100,000xg离心分离的上清液部份或酚提取物,连续四天注射于正常小鼠腹腔,能使小鼠肝的
The isoenzyme patterns of pyruvate kinases extracted from mouse tissue extracts using isoelectric focusing separation technology were found to be very similar to those of rat tissues. However, isoelectric point focusing separation analysis or kinetic analysis of extracts of normal mouse liver and Ehrlich ascites carcinoma host liver showed that the latter had a higher proportion of pyruvate kinase K isozyme. The supernatant or the phenol extract of Ehrlich ascites carcinoma cell homogenate was centrifuged at 100,000 x g for 4 consecutive days in the abdominal cavity of normal mice to make the mice liver